TIRF Microscopy for the Study of Nanobubble Dynamics
نویسندگان
چکیده
Nanobubbles can be observed with optical microscopy using the total internal re ection uorescence (TIRF) excitation. We report on TIRF visualization using Rhodamine 6G at 5μM concentration which results to strongly contrasted pictures. The preferential absorption and the high spatial resolution allow to detect nanobubbles with diameters of 230 nm and above. We resolve the nucleation dynamics during the water-ethanol-water exchange: Within 4 min after the exchange bubble nucleate and form a stable population. Additionally, we demonstrate that tracer particles near to the nanobubbles are following Brownian motion: A remaining drift ow is weaker than a few micrometers per second at a distance of 400 nm from the nanobubble's center.
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